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Immunohistochemical staining of human smooth muscle shows distinct cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows distinct cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows distinct cytoplasmic positivity in smooth muscle cells.

Anti-BAG2 Antibody

HPA018862
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBAG2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BAG2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9532
  • Target name
    BAG2
  • Target description
    BAG cochaperone 2
  • Target synonyms
    BAG family molecular chaperone regulator 2; BAG-2; BCL2 associated athanogene 2; bcl-2-associated athanogene 2; dJ417I1.2; dJ417I1.2 (BAG-family molecular chaperone regulator 2)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95816
  • Protein Name
    BAG family molecular chaperone regulator 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of BAG2 using anti-BAG2 antibody (PB9524). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAG2 antigen affinity purified polyclonal antibody (Catalog # PB9524) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAG2 at approximately 24 kDa. The expected band size for BAG2 is at 24 kDa.
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