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BBS9 antibody

FNAB00825
Wuhan Fine Biotech
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetBBS9
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Overview

  • Supplier
    Wuhan Fine Biotech
  • Product Name
    BBS9 antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Estimated Purity
    ≥95%
  • Gene ID27241
  • Target name
    BBS9
  • Target description
    Bardet-Biedl syndrome 9
  • Target synonyms
    B1, C18, D1, PTHB1, protein PTHB1, PTH-responsive osteosarcoma B1 protein, bardet-Biedl syndrome 9 protein, parathyroid hormone-responsive B1 gene protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ3SYG4
  • Protein Name
    Protein PTHB1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BBS9 using anti-BBS9 antibody (A07367-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BBS9 antigen affinity purified polyclonal antibody (Catalog # A07367-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BBS9 at approximately 99 kDa. The expected band size for BBS9 is at 99 kDa.
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