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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Anti-BBS9 Antibody

HPA021289
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBBS9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BBS9
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27241
  • Target name
    BBS9
  • Target description
    Bardet-Biedl syndrome 9
  • Target synonyms
    B1; bardet-Biedl syndrome 9 protein; C18; D1; parathyroid hormone-responsive B1 gene protein; protein PTHB1; PTHB1; PTH-responsive osteosarcoma B1 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ3SYG4
  • Protein Name
    Protein PTHB1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of BBS9 using anti-BBS9 antibody (A07367-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BBS9 antigen affinity purified polyclonal antibody (Catalog # A07367-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BBS9 at approximately 99 kDa. The expected band size for BBS9 is at 99 kDa.
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