Anti-BCMA [17A5]

Absolute Antibody

Anti-BCMA [17A5]

7

The selection of specific binders having affinity for BCMA was done using ELISA and their affinity was determined using SPR analysis. The original Fab antibody bound human and mouse BCMA with a monovalent binding affinity of 45 nM and 74 nM respectively. The binding of the IgG version of this antibody to membrane bound human BCMA was determined by performing flow cytometry on BCMA-expressing H929 cells and MKN45 cells. The assessment of the competition between A-APRIL and this antibody was performed on H929 cells by quantifying the binding of A-APRIL in presence of increasing concentrations of this antibody and measured by flow cytometry. This antibody was reported to not block or increase APRIL-dependent NF-kappaB activation and also does not block or increase BAFF-dependent NF-kappaB activation in vitro. This antibody was also reported to be used in the generation of antibody-drug conjugates and anti-BCMA/anti-CD3 T cell bispecific antibodies for possible immunotherapy applications in treatment of multiple myelomas (EP2953972).

Flow Cytometry, ELISA

ELISA; FC

Research Use Only

Tumor necrosis factor receptor superfamily member 17

This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203