Anti-beta 1 integrin [AIIB2]

Absolute Antibody

Anti-beta 1 integrin [AIIB2]

7

The antibody specifically binds beta1 integrin extracellular domain.

The antibody immunoprecipitated the integrin subunits (Hall et al.,1990). This antibody was used for detection of beta1 integrin expressed on CHO cells by flow cytometry (Takada et al., 1993; PMID: 7688727). The original format of the antibody or its F(ab)2 fragments applied to single cells were capable of down-modulating beta1 integrin signaling pathways (Wang et al., 1998; PMID: 9843973 and Weaver et al., 1997; PMID: 9105051). The antibody was used in immunofluorescence characterization of integrins in the HMT-3522 cells (Weaver et al., 1997; PMID: 9105051). The antibody inhibited tumor cell growth in several breast cancer cell lines (T4-2, MDA-MB-231, BT474, SKBR3, and MCF-7) and one nonmalignant cell line (S-1). Further, beta1 integrin inhibition resulted in a significant loss of cancer cells, associated with a decrease in proliferation and increase in apoptosis, and a global change in the composition of residual colonies (Park et al., 2010; PMID: 16452209). The antibody was found to reduce the frequency of solid tumor stem cells. The antibody inhibited the growth of colon tumors and reduced the percentage of CD44+ cells. Tumors treated in mice showed a decrease when treated with the antibody. The humanized version of the antibody was constructed, showing potential for therapeutic uses (WO2009009114A3).

Flow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking, Other Application

in vivo; inhibition; FACS; IP; FC

Research Use Only

Monoclonal

AIIB2

ITGB1BP1

integrin subunit beta 1 binding protein 1

bodenin; ICAP1; ICAP1A; ICAP-1A; ICAP-1alpha; ICAP1B; ICAP-1B; integrin beta-1-binding protein 1; integrin cytoplasmic domain-associated protein 1; integrin cytoplasmic domain-associated protein 1-alpha; integrin cytoplasmic domain-associated protein 1-beta

Rabbit

IgG

Integrin beta-1-binding protein 1

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format for improved compatibility with existing reagents assays and techniques.

Human, Mouse

Human, mouse

The original antibody was isolated by immunizing a Lewis rat with JAR choriocarcinoma cells.

-20°C,2°C to 8°C

12352203