Anti-beta tubulin type III [5G8]

Absolute Antibody

Anti-beta tubulin type III [5G8]

7

This antibody is specific for amino acids 436-450 of beta tubulin type III. IT binds to human, mice and rat beta tubulin type III. Its exact epitope is EAQGPK. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchange

The mouse version of this antibody was used for a western blot on purified beta tubulin type III (Spano and Frankfurter, 2010; pmid:20466128). Neuronal cultures of leucine-rich repeat kinase 2 knock-out mice were immunostained using the mouse version of this antibody (Gillardon, 2009; pmid:19545277). To determine selective expression of beta-tubulin isotypes in taxane resistance, immunohistochemistry was preformed on human primary ovarian carcinoma tissues using the mouse version of this antibody (Ohishi et al, 2007; pmid:17343904). While determining the localization of betaIII-tubulin in colorectal cancer specimens, the mouse version of this antibody was used for immunohistochemistry on colorectal cancer tissue (Portyanko et al, 2009; pmid:19360438). To investigate the protein antigens targeted by antibody diversity of infected mice with Trypanosoma cruzi, immunoblots were preformed against recombinant TcbetaTUB (Montalvao et al, 2018; pmid:29706955).

ImmunoFluorescence, Western Blot, ImmunoHistoChemistry

WB; IF; IHC

Research Use Only

Monoclonal

5G8

TUBB3

tubulin beta 3 class III

beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; class III beta-tubulin; FEOM3; TUBB4; tubulin beta-3 chain; tubulin beta-4 chain; tubulin beta-III; tubulin, beta 3

Human

IgG1

Tubulin beta-3 chain

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human, Mouse, Rat

Human, Mouse, Rat

The mouse version of this antibody was generated by immunizing mice with a synthetic peptide corresponding to the C-terminus of human beta III tubulin.

-20°C,2°C to 8°C

12352203