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Immunohistochemistry analysis in human placenta and skeletal muscle tissues using HPA003157 antibody. Corresponding BGN RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human placenta and skeletal muscle tissues using HPA003157 antibody. Corresponding BGN RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human placenta and skeletal muscle tissues using HPA003157 antibody. Corresponding BGN RNA-seq data are presented for the same tissues.

Anti-BGN Antibody

HPA003157
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBGN
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BGN Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID633
  • Target name
    BGN
  • Target description
    biglycan
  • Target synonyms
    DSPG1, MRLS, PG-S1, PGI, SEMDX, SLRR1A, biglycan, bone/cartilage proteoglycan-I, dermatan sulphate proteoglycan I, small leucine-rich protein 1A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21810
  • Protein Name
    Biglycan
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Biglycan/BGN using anti-Biglycan/BGN antibody (A02090-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COLO320 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HUVEC whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat L6 whole cell lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Biglycan/BGN antigen affinity purified polyclonal antibody (Catalog # A02090-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Biglycan/BGN at approximately 48 kDa. The expected band size for Biglycan/BGN is at 42 kDa.
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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Biglycan Polyclonal Antibody (bs-7552R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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