Figure 3. IHC analysis of IBSP using anti-IBSP antibody (PA1887). IBSP was detected in paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-IBSP Antibody (PA1887) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of IBSP using anti-IBSP antibody (PA1887). IBSP was detected in paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-IBSP Antibody (PA1887) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Bone Sialoprotein/IBSP Antibody Picoband(r)
PA1887-DYLIGHT550
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetIbsp
Overview
- SupplierBoster Bio
- Product NameAnti-Bone Sialoprotein/IBSP Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for IBSP is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateDyLight 550
- Gene ID15891
- Target nameIbsp
- Target descriptionintegrin binding sialoprotein
- Target synonymsB; bone sialoprotein 2; bone sialoprotein II; Bs; BSP; BSP II; Bsp2; BSPII; cell-binding sialoprotein
- HostRabbit
- IsotypeIgG
- Protein IDQ61711
- Protein NameBone sialoprotein 2
- Scientific DescriptionBoster Bio Anti-Bone Sialoprotein/IBSP Antibody catalog # PA1887. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203