Anti-Botulinum neurotoxin [BotFab 5]

Absolute Antibody

Anti-Botulinum neurotoxin [BotFab 5]

7

The Fab was shown by ELISA to be reactive to botulinum types A and B toxin complexes and not reactive to the 150 kDa NT of type A or B that lacked the non-NT proteins found in the complex. (US5932449A) Surface plasmon resonance demonstrated that the Fab displays a slightly tighter equilibrium binding constant towards botulinum toxin B than toxin A. The dissociation rate constants (kd1 and kd2) with botulinum toxin A are 7.1x10^-3 and 2.3x10^-4 s^-1 (ratio 1:2), while with botulinum toxin B the values are 3.7x10^-3 and 5.7x10^-4 s^-1 (ratio 1:3.5). The Fab antibody was used in an indirect flow cytometric immunoassay to detect the presence of pentavalent toxoid A-B-C-D-E and botulinum toxoid A; the antibody detected the presence of botulinum toxoid A. The limit of sensitivity of the Fab fragment for botulinum toxoid is between 5 and 10 ng/ml (PMID: 10945449)

Flow Cytometry, ELISA, Other Application

ELISA; SPR; FC

Research Use Only

Botulinum neurotoxin type B

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203