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Immunohistochemical staining of human stomach cancer shows moderate nuclear positivity in tumor cells.
Immunohistochemical staining of human stomach cancer shows moderate nuclear positivity in tumor cells.
Immunohistochemical staining of human stomach cancer shows moderate nuclear positivity in tumor cells.

Anti-BRD4 Antibody

AMAB90841
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBRD4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BRD4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL1115
  • Conjugate
    Unconjugated
  • Gene ID23476
  • Target name
    BRD4
  • Target description
    bromodomain containing 4
  • Target synonyms
    CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP, bromodomain-containing protein 4, chromosome-associated protein, female sterile homeotic related gene 4, mitotic chromosome-associated protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO60885
  • Protein Name
    Bromodomain-containing protein 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of BRD4 using anti-BRD4 antibody (A00123-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD4 antigen affinity purified polyclonal antibody (Catalog # A00123-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD4 at approximately 180 kDa. The expected band size for BRD4 is at 152 kDa.
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