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Immunohistochemical staining of human Cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human Cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human Cerebral cortex shows strong nuclear positivity in neuronal cells.

Anti-BRD9 Antibody

HPA023197
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBRD9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BRD9 Antibody
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID65980
  • Target name
    BRD9
  • Target description
    bromodomain containing 9
  • Target synonyms
    LAVS3040, PRO9856, SMARCI2, bromodomain-containing protein 9, rhabdomyosarcoma antigen MU-RMS-40.8, sarcoma antigen NY-SAR-29
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H8M2
  • Protein Name
    Bromodomain-containing protein 9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of BRD9 using anti-BRD9 antibody (A08420-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD9 antigen affinity purified polyclonal antibody (Catalog # A08420-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD9 at approximately 80 kDa. The expected band size for BRD9 is at 67 kDa.
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