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ProductsBack/Anti-Butyrylcholinesterase [11D8]

Anti-Butyrylcholinesterase [11D8]

Ab00765-1.1
Absolute Antibody
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetBCHE
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Butyrylcholinesterase  [11D8]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The percent active HuBChE bound to 11D8 in ELISAS is highest when the volume of plasma was lowest. 11D8 also binds to human albumin and so 11D8 is less suitable for immunopurification of huBChE from plasma compared to e.g. B2 18-5.
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA; IP; IHC; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    11D8
  • Gene ID590
  • Target name
    BCHE
  • Target description
    butyrylcholinesterase
  • Target synonyms
    BCHED, CHE1, CHE2, E1, cholinesterase, acylcholine acylhydrolase, butyrylcholine esterase, choline esterase II, cholinesterase (serum) 2, cholinesterase 1, pseudocholinesterase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP06276
  • Protein Name
    Cholinesterase
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    11D8 wsa prepared by immunizing BChE -/- mice with sugar-diminished full-length recombinant human BChE (Hrabovska et al. 2010).
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCHE using anti-BCHE antibody (A00258-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCHE antigen affinity purified polyclonal antibody (Catalog # A00258-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCHE at approximately 85-90 kDa. The expected band size for BCHE is at 68 kDa.
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