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Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.

Anti-C7 Antibody

HPA001465
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetC7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-C7
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID730
  • Target name
    C7
  • Target description
    complement C7
  • Target synonyms
    complement component C7, complement component 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10643
  • Protein Name
    Complement component C7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Complement C7 using anti-Complement C7 antibody (A00844-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 293T cell lysates, Lane 2: human HepG2 cell lysates, Lane 3: human A549 cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Complement C7 antigen affinity purified polyclonal antibody (Catalog # A00844-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Complement C7 at approximately 93KD. The expected band size for Complement C7 is at 93KD.
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