Figure 1. IHC analysis of Calcitonin using anti-Calcitonin antibody (PB9936). Calcitonin was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Calcitonin Antibody (PB9936) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Calcitonin using anti-Calcitonin antibody (PB9936). Calcitonin was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Calcitonin Antibody (PB9936) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-Calcitonin/CALCA Antibody
PB9936-CARRIER-FREE
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetCALCA
Overview
- SupplierBoster Bio
- Product NameAnti-Calcitonin/CALCA Antibody
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID796
- Target nameCALCA
- Target descriptioncalcitonin related polypeptide alpha
- Target synonymsCALC1, CGRP, CGRP-I, CGRP-alpha, CGRP1, CT, KC, PCT, calcitonin, calcitonin gene-related peptide 1, alpha-type CGRP, calcitonin 1, calcitonin gene-related peptide I, calcitonin/calcitonin-related polypeptide, alpha, katacalcin
- HostRabbit
- IsotypeIgG
- Protein IDP06881
- Protein NameCalcitonin gene-related peptide 1
- Scientific DescriptionBoster Bio Anti-Calcitonin/CALCA Antibody Picoband® catalog # PB9936. Tested in IHC applications. This antibody reacts with Human.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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