Anti-calf thymus ATPaseA [4B10-A3-E4]

Absolute Antibody

Anti-calf thymus ATPaseA [4B10-A3-E4]

9

This antibody is specific for calf thymus ATPase A and recognizes bovine, human, and mouse homologs. Calf ATPaseA is a TP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably

The mouse version of this antibody was used for a western blot on calf DNA-dependent ATPase A isolated from calf thymus using immunoprecipitation (Mesner et al, 1993; pmid:8394114). To identify the mouse version of this antibody an ELISA on native ATPase A was preformed. Furthermore, a western blot was preformed on bacteriophage T4 gene 44/62 and 45 proteins. It showed a strong reaction (Mesner et al, 1991; pmid:1720977). The ATPase A activity under either Ca2+-activated or Mg3+-actin-activated conditions were determined in the presence of this antibody. No change in activity was noticed (Eldin et al, 1993; pmid:8448113).

Functional Assay, Western Blot, ELISA

Research Use Only

Monoclonal

4B10-A3-E4

Mouse

IgG1

Bovine

-20°C,2°C to 8°C

12352203