Anti-Calmodulin-domain protein kinase 1 [VHH-1B7]

Absolute Antibody

Anti-Calmodulin-domain protein kinase 1 [VHH-1B7]

9

CDPKs regulate essential processes in parasites.VHH-IB7 recognises CDPK1 in a conformation-dependent manner and selectively inhibits the Ca^2+ bound form. It also recognises CDPK3 in a Ca2+-bound form.

ELISA showed VHH-1B7 has reactivity against CDPK1. By immunoblot, 1B7 recognized both endogenous and recombinant versions of CDPK1 and its closest paralog CDPK3 (Ingram et al, 2015). Immunoprecipitation showed VHH-1B7 specifically recognises the Ca2+-bound form of CDPK1 and CDPK3. VHH-1B7 inhibits CDPK1 and CDPK3 with an IC50 of approximately 40 nm. The crystal structure of CDPK1 with VHH-1B7 showed VHH-1B7 interacts directly with the regulatory domain of CDPK1.

ImmunoPrecipitation, ELISA, Other Application

Research Use Only

Monoclonal

VHH-1B7

Human

IgG1

This chimeric human antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Parasite

-20°C,2°C to 8°C

12352203