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Western blot analysis using anti-Calreticulin (human), pAb (Prod. No. AG-25A-0094) at 1:4000 dilution. 1: Human Calreticulin (his-tagged). 2: HEK 293T cell lysate (100microg). 3: HepG2 cell lysate (100microg). 4: THP1 cell lysate (1
Western blot analysis using anti-Calreticulin (human), pAb (Prod. No. AG-25A-0094) at 1:4000 dilution. 1: Human Calreticulin (his-tagged). 2: HEK 293T cell lysate (100microg). 3: HepG2 cell lysate (100microg). 4: THP1 cell lysate (1
Western blot analysis using anti-Calreticulin (human), pAb (Prod. No. AG-25A-0094) at 1:4000 dilution. 1: Human Calreticulin (his-tagged). 2: HEK 293T cell lysate (100microg). 3: HepG2 cell lysate (100microg). 4: THP1 cell lysate (1

anti-Calreticulin (human), pAb

AG-25A-0094
AdipoGen Life Sciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCALR
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-Calreticulin (human), pAb
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID811
  • Target name
    CALR
  • Target description
    calreticulin
  • Target synonyms
    calregulin; calreticulin; cC1qR; CRP55; CRT; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; grp60; HACBP; HEL-S-99n; RO; Sicca syndrome antigen A (autoantigen Ro; calreticulin); SSA
  • Host
    Rabbit
  • Protein IDP27797
  • Protein Name
    Calreticulin
  • Scientific Description
    Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the calreticulin/calnexin cycle that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule. - Polyclonal Antibody. Recognizes human calreticulin. Detects a band of ~60kDa by Western blot. Source: Rabbit. Applications: ELISA, WB. Liquid. 0.2microm-filtered solution in PBS, pH 7.4. Contains no preservatives. Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the calreticulin/calnexin cycle that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CALR using anti-CALR antibody (A00894-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CALR antigen affinity purified polyclonal antibody (Catalog # A00894-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CALR at approximately 60KD. The expected band size for CALR is at 48KD.
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