Anti-Capsid protein VP25 [PL-2 scFv], Mouse IgG1, kappa

Absolute Antibody

Anti-Capsid protein VP25 [PL-2 scFv], Mouse IgG1, kappa

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This antibody is specific for Capsid protein VP25 from Human astrovirus-2.

The structure of the original antibody was determined using x-ray crystallography. This antibody was used for a western blot on HAstV-2 capsid protein (Bogdanoff et al., 2016; PMID:27213181). The original antibody was used for an ELISA on purified Spike or GFP-Spike from HAstV-2 (Bogdanoff et al., 2017; PMID:27807234, Bogdanoff et al., 2018; PMID:29070688), and on purified Human astrovirus-2 (Sanchez-Fauquier et al., 1994; PMID:7514320). Further this antibody was used for surface plasmon resonance on Spike2 from HAstV-2. This antibody has shown that it is able to block the GFP-Spike2 binding to Caco-2 cells (Bogdanoff et al., 2017; PMID:27807234). This antibody was used for immunofluorescence on Human astrovirus-2 infected LLCMK2. This antibody was used for radioimmunoprecipitation on virus samples (Sanchez-Fauquier et al., 1994; PMID:7514320). This antibody was used for a neutralization assay by preincubating human astrovirus-2 with this antibody and then adding C2Bbe1 cells (Bogdanoff et al., 2018; PMID:29070688).

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

PL-2 scFv

Mouse

IgG1

Capsid polyprotein VP90

Virus

-20°C,2°C to 8°C

12352203