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Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.

Anti-CARM1 Antibody

HPA048073
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCARM1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CARM1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10498
  • Target name
    CARM1
  • Target description
    coactivator associated arginine methyltransferase 1
  • Target synonyms
    PRMT4, histone-arginine methyltransferase CARM1, protein arginine N-methyltransferase 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86X55
  • Protein Name
    Histone-arginine methyltransferase CARM1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue, Mouse liver tissue, Mouse kidney tissue All lanes: CARM1 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66, 64, 42 kDa Observed band size: 66, 42 kDa
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Figure 1. Western blot analysis of CARM1 using anti-CARM1 antibody (A01486-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARM1 antigen affinity purified polyclonal antibody (Catalog # A01486-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARM1 at approximately 66 kDa. The expected band size for CARM1 is at 66 kDa.
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