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Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes.

Anti-CASP5 Antibody

HPA040937
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCASP5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CASP5
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID838
  • Target name
    CASP5
  • Target description
    caspase 5
  • Target synonyms
    ICE(rel)III, ICEREL-III, ICH-3, caspase-5, TY protease, caspase 5, apoptosis-related cysteine peptidase, caspase 5, apoptosis-related cysteine protease, protease ICH-3, protease TY
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51878
  • Protein Name
    Caspase-5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Caspase-5/CASP5 using anti-Caspase-5/CASP5 antibody (A05259-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-5/CASP5 antigen affinity purified polyclonal antibody (Catalog # A05259-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-5/CASP5 at approximately 50KD. The expected band size for Caspase-5/CASP5 is at 50KD.
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