Western blot analysis using anti-caspase-12 (mouse), mAb (12G6) (Prod. No. AG-20T-0141) detecting, A) endogenous caspase-12 in mouse lung, liver, spleen and pancreas, and B) pro-caspase-12 in mouse MEFs.
Western blot analysis using anti-caspase-12 (mouse), mAb (12G6) (Prod. No. AG-20T-0141) detecting, A) endogenous caspase-12 in mouse lung, liver, spleen and pancreas, and B) pro-caspase-12 in mouse MEFs.
anti-Caspase-12 (mouse), mAb (12G6)
AG-20T-0141
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetCasp12
Overview
- SupplierAdipoGen Life Sciences
- Product Nameanti-Caspase-12 (mouse), mAb (12G6)
- Delivery Days Customer10
- Antibody SpecificityRecognizes an epitope within the p20 fragment of mouse caspase-12. Detects a band of ~51kDa by Western blot. Does not cross-react with mouse caspase-1 or -11.
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID12G6
- Concentration1 mg/ml
- Estimated Purity>95%
- FormulationLiquid
- Gene ID12364
- Target nameCasp12
- Target descriptioncaspase 12
- Target synonymsCASP-12; caspase-12
- HostRat
- IsotypeIgG1
- Protein IDO08736
- Protein NameCaspase-12
- Scientific DescriptionCaspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed. - Monoclonal Antibody. Recognizes an epitope within the p20 fragment of mouse caspase-12. Detects a band of ~51kDa by Western blot. Does not cross-react with mouse caspase-1 or -11. Isotype: Rat IgG1kappa. Clone: 12G6. Applications: ELISA, FACS, WB. Liquid. In PBS containing 0.02% sodium azide. Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed.
- ReactivityMouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203