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Immunohistochemical staining of human skeletal muscle shows cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows cytoplasmic positivity in myocytes.

Anti-CAST Antibody

HPA036881
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCAST
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CAST Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID831
  • Target name
    CAST
  • Target description
    calpastatin
  • Target synonyms
    BS-17, MIR583HG, PLACK, calpastatin, MIR583 host, calpain inhibitor, sperm BS-17 component
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20810
  • Protein Name
    Calpastatin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Calpastatin using anti-Calpastatin antibody (A00337). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpastatin antigen affinity purified polyclonal antibody (Catalog # A00337) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calpastatin at approximately 120 kDa. The expected band size for Calpastatin is at 77 kDa.
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