Anti-Cathepsin B [2A2]

Absolute Antibody

Anti-Cathepsin B [2A2]

9

This antibody binds human cathepsin B (Cat B) at an epitope near the active site and inhibits its proteolytic activity. It was reported to specifically bind the EPGYSP sequence located between amino acid residues 133-138 of cathepsin B. Cat B is a lysosom

This antibody is capable of binding cathepsin B in an antigen-immobilized ELISA. The binding reactivity of this antibody to cathepsin B was also confirmed using Western blot. This antibody significantly inhibited the invasion of ras-transformed MCF-10A neoT cells through matrigel. This antibody was further reported to inhibit cathepsin B activity against BODYPY FL casein significantly (Fan et al., 2002; PMID: 12530548). In a study, the intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT, were assessed using this antibody. This antibody was shown to impair both the intracellular and the extracellular fractions of cathepsin B activity (Premzl et al., 2003; PMID: 12581740). This antibody was also used in the generation of an ammodytoxin-anti-cathepsin B immuno-conjugate to deliver secretory phospholipase A2 into cancerous cells (Premzl et al., 2008; PMID: 18221975). This antibody was reported to potentiate cathepsin B exopeptidase activity with an activation constant (Ka) of 22.3 nm, although simultaneously inhibiting its endopeptidase activity (Mirkovi et al., 2009; PMID: 19656187).

Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

2A2

CTSB

cathepsin B

amyloid precursor protein secretase; APP secretase; APPS; cathepsin B; cathepsin B1; CPSB; cysteine protease; epididymis secretory sperm binding protein; RECEUP

Mouse

IgG1

Cathepsin B

Human

-20°C,2°C to 8°C

12352203