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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Anti-CBX2 Antibody

HPA023083
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCBX2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CBX2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84733
  • Target name
    CBX2
  • Target description
    chromobox 2
  • Target synonyms
    CDCA6; cell division cycle associated 6; chromobox homolog 2 (Pc class homolog, Drosophila); chromobox protein homolog 2; M33; modifier 3; Pc class homolog; SRXY5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14781
  • Protein Name
    Chromobox protein homolog 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CBX2 using anti-CBX2 antibody (A06356-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBX2 antigen affinity purified polyclonal antibody (Catalog # A06356-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBX2 at approximately 56 kDa. The expected band size for CBX2 is at 56 kDa.
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