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Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Anti-CCDC88A Antibody

HPA038101
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCCDC88A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CCDC88A
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55704
  • Target name
    CCDC88A
  • Target description
    coiled-coil and HOOK domain protein 88A
  • Target synonyms
    APE, GIRDIN, GIV, GRDN, HkRP1, KIAA1212, PEHO, PEHOL, girdin, AKT-phosphorylation enhancer, Hook-related protein 1, coiled-coil domain containing 88A, g alpha-interacting vesicle-associated protein, girders of actin filaments
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ3V6T2
  • Protein Name
    Girdin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of CCDC88A using anti-CCDC88A antibody (A03282-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC88A antigen affinity purified polyclonal antibody (Catalog # A03282-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC88A at approximately 240 kDa. The expected band size for CCDC88A is at 216 kDa.
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