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Anti-CCL16 Antibody

130-10886-100
RayBiotech
ApplicationsELISA
Product group Antibodies
TargetCCL16
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-CCL16 Antibody
  • Delivery Days Customer
    16
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5B7-A12-A1
  • Gene ID6360
  • Target name
    CCL16
  • Target description
    C-C motif chemokine ligand 16
  • Target synonyms
    CKb12, HCC-4, ILINCK, LCC-1, LEC, LMC, Mtn-1, NCC-4, NCC4, SCYA16, SCYL4, C-C motif chemokine 16, IL-10-inducible chemokine, chemokine (C-C motif) ligand 16, chemokine LEC, liver CC chemokine-1, liver-expressed chemokine, lymphocyte and monocyte chemoattractant, monotactin-1, new CC chemokine 4, small inducible cytokine subfamily A (Cys-Cys), member 16, small-inducible cytokine A16
  • Host
    Mouse
  • Protein IDO15467
  • Protein Name
    C-C motif chemokine 16
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCL16 using anti-CCL16 antibody (A07545-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL16 antigen affinity purified polyclonal antibody (Catalog # A07545-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL16 at approximately 18KD. The expected band size for CCL16 is at 14KD.
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