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Anti-CCL18 [AZN-CK18]

AB01647-1.1-BT
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCCL18
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CCL18 [AZN-CK18]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody can be used both in direct and sandwich ELISA (van der Voort et al., 2005). The antibody was shown to work in immunohistochemistry (Lindhout et al, 2001). Immunopercipitation can be done using this antibody (van der Voort et al., 2005). The antibody is also reccomended for flow cytometry.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AZN-CK18
  • Gene ID6362
  • Target name
    CCL18
  • Target description
    C-C motif chemokine ligand 18
  • Target synonyms
    alternative macrophage activation-associated CC chemokine 1; AMAC1; AMAC-1; CC chemokine ligand 18; CC chemokine PARC; C-C motif chemokine 18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); chemokine (C-C), dendritic; CKb7; DCCK1; DC-CK1; dendritic cell chemokine 1; macrophage inflammatory protein 4; MIP-4; PARC; pulmonary and activation-regulated chemokine; SCYA18; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP55774
  • Protein Name
    C-C motif chemokine 18
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCL18 using anti-CCL18 antibody (PB9023). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human CCL18 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL18 antigen affinity purified polyclonal antibody (Catalog # PB9023) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL18 at approximately 19KD. The expected band size for CCL18 is at 19KD.
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