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Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.

Anti-CCR6 Antibody

HPA014488
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCCR6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CCR6
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1235
  • Target name
    CCR6
  • Target description
    C-C motif chemokine receptor 6
  • Target synonyms
    BN-1; C-C chemokine receptor type 6; C-C CKR-6; CC-CKR-6; CCR-6; CD196; chemokine (C-C motif) receptor 6; chemokine (C-C) receptor 6; chemokine receptor-like 3; CKRL3; CKR-L3; CMKBR6; DCR2; DRY6; G protein-coupled receptor 29; GPR29; GPRCY4; LARC receptor; seven-transmembrane receptor, lymphocyte, 22; STRL22
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51684
  • Protein Name
    C-C chemokine receptor type 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CCR6 Polyclonal Antibody, Unconjugated (bs-1542R) at 1:200 overnight at 4°C, followed by a conjugated secondary (bs-0295G-AF594) at [1:500] for 90 minutes and DAPI staining of the nuclei.
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Figure 1. IHC analysis of CCR6 using anti-CCR6 antibody (A00957). CCR6 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-CCR6 Antibody (A00957) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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