Anti-CCR7 [4B12]

Absolute Antibody

Anti-CCR7 [4B12]

7

This antibody is specific to the mouse CCR7 protein, which was confirmed by immunoprecipitation (Ritter et al, 2004).

This antibody was used for flow cytometry and the staining quality can be improved by incubation at 37°C (Britschgi et al, 2008). This antibody is widely used to monitor the activation of dendritic cells in mice (Ohl et al, 2004; Ritter et al, 2004; Zhao et al, 2019). It was also utilized in immunoprecipitation of the CCR7 receptor protein (Ritter et al, 2004). The antibody does not interfere with ligands binding to the CCR7 receptor, which was confirmed by a transmigration assay (Ritter et al, 2004). It does not interfere with T-cell migration (Ritter et al, 2004). The antibody also is not agonistic as it does not activate the receptor (Ritter et al, 2004).

Functional Assay, Flow Cytometry, ImmunoPrecipitation

FC; IP; functional assays

Research Use Only

Monoclonal

4B12

Ccr7

chemokine (C-C motif) receptor 7

C-C chemokine receptor type 7; CC-CKR-7; CCR-7; CD197; Cdw197; chemokine (C-C) receptor 7; Cmkbr; Cmkbr7; EBI; EBI1; Ebi1h; EBV-induced G-protein coupled receptor 1; epstein-Barr virus-induced G-protein coupled receptor 1; MIP-3 beta receptor

Mouse

IgG2b

C-C chemokine receptor type 7

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

This antibody was raised by immunizing rats with mouse CCR7 antigen.

-20°C,2°C to 8°C

12352203