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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.

Anti-CCS Antibody

HPA044090
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCCS
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CCS Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9973
  • Target name
    CCS
  • Target description
    copper chaperone for superoxide dismutase
  • Target synonyms
    copper chaperone for superoxide dismutase, superoxide dismutase copper chaperone
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14618
  • Protein Name
    Copper chaperone for superoxide dismutase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCS using anti-CCS antibody (A00314-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, Lane 2: rat spleen tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCS antigen affinity purified polyclonal antibody (Catalog # A00314-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCS at approximately 34KD. The expected band size for CCS is at 29KD.
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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.
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