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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Anti-CCT8 Antibody

HPA021051
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCCT8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CCT8 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10694
  • Target name
    CCT8
  • Target description
    chaperonin containing TCP1 subunit 8
  • Target synonyms
    C21orf112, Cctq, D21S246, PRED71, T-complex protein 1 subunit theta, CCT-theta, TCP-1-theta, chaperonin containing T-complex polypeptide 1 subunit 8, chaperonin containing TCP1, subunit 8 (theta), renal carcinoma antigen NY-REN-15
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50990
  • Protein Name
    T-complex protein 1 subunit theta
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Western Blot Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate All lanes: CCT8 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 58, 52 kDa Observed band size: 60 kDa
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Figure 1. Western blot analysis of CCT8 using anti-CCT8 antibody (PB9993). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Colo320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT8 antigen affinity purified polyclonal antibody (Catalog # PB9993) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT8 at approximately 60 kDa. The expected band size for CCT8 is at 60 kDa.
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References
GeneTex
TCP1 theta antibody [N2C2], InternalGTX105725
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCCT8
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