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Anti-CD117 [33G5]

Ab03319-10.0
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetKIT
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD117 [33G5]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The antibody specifically detected CD117 protein in Western blot analysis. The antibody 33G5 and the commercial antibody YR145 were used to stain gastrointestinal stromal tumor tissue. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 33G5 was higher than the commercial antibody. The antibody and the commercially available antibody YR145 were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN113621064A).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    IHC; WB; ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    33G5
  • Gene ID3815
  • Target name
    KIT
  • Target description
    KIT proto-oncogene, receptor tyrosine kinase
  • Target synonyms
    CD117; C-Kit; c-Kit protooncogene; mast/stem cell growth factor receptor Kit; MASTC; p145 c-kit; PBT; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP10721
  • Protein Name
    Mast/stem cell growth factor receptor Kit
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing mice with CD117.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. IHC analysis of Kit using anti-Kit antibody (A01335-1). Kit was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Kit Antibody (A01335-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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