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ProductsBack/Anti-CD127 [1A11]

Anti-CD127 [1A11]

Ab02640-1.1
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, Other Application
Product group Antibodies
TargetIL7R
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD127 [1A11]
  • Delivery Days Customer
    7
  • Application Supplier Note
    ELISA was performed on CD127 using this antibody (WO2010017468). This antibody was tested for inhibition of IL-7 induced IFN-gamma and IL-17 in a TH17 expansion assay (WO2010017468). Surface plasmon resonance was used to assess the ability of this antibody to bind Fc effector receptors (Fc Gamma I, IIa and IIIa) (US9150653). Surface plasmon resonance was performed with this antibody on CD127 to test the binding affinity (WO2010017468). Immunohistochemistry was performed on the spinal cord of a mouse using this antibody (WO2010017468). Western blot was performed on protein extract using this antibody (WO2010017468).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, Other Application
  • Applications Supplier
    antagonist; ELISA; IHC; SPR; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1A11
  • Gene ID3575
  • Target name
    IL7R
  • Target description
    interleukin 7 receptor
  • Target synonyms
    CD127; CD127 antigen; CDW127; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL7RA; IL-7R-alpha; ILRA; interleukin 7 receptor alpha chain; interleukin-7 receptor subunit alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP16871
  • Protein Name
    Interleukin-7 receptor subunit alpha
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing Balb/c mice with the full ECD of CD127.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD127/IL7R using anti-CD127/IL7R antibody (A02222-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD127/IL7R antigen affinity purified polyclonal antibody (Catalog # A02222-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD127/IL7R at approximately 170 kDa. The expected band size for CD127/IL7R is at 52 kDa.
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