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Immunohistochemical staining of formalin fixed and paraffin embedded breast ductal carcinoma section using Anti-Foxp1 Rabbit Monoclonal Antibody (Clone RM402) at a 1:200 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded breast ductal carcinoma section using Anti-Foxp1 Rabbit Monoclonal Antibody (Clone RM402) at a 1:200 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded breast ductal carcinoma section using Anti-Foxp1 Rabbit Monoclonal Antibody (Clone RM402) at a 1:200 dilution.

anti-CD13 (human), Rabbit Monoclonal (RM403)

REV-31-1289-00
RevMAb Biosciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetANPEP
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Overview

  • Supplier
    RevMAb Biosciences
  • Product Name
    anti-CD13 (human), Rabbit Monoclonal (RM403)
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RM403
  • Gene ID290
  • Target name
    ANPEP
  • Target description
    alanyl aminopeptidase, membrane
  • Target synonyms
    alanyl (membrane) aminopeptidase; aminopeptidase M; aminopeptidase N; AP-M; APN; AP-N; CD13; GP150; hAPN; LAP1; membrane alanyl aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13; P150; PEPN
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15144
  • Protein Name
    Aminopeptidase N
  • Scientific Description
    CD13 (ANPEP) is a 150-170 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. CD13 is a metalloprotease that preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 also has a role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is expressed by granulocytes, monocytes, myeloid leukemia cells and is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas. CD13 functions as an aminopeptidase enzyme and is also alpha receptor for coronavirus an important cause of upper respiratory tract infections. - Recombinant Antibody. This antibody reacts to human to CD13. Applications: WB, IHC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. CD13 (ANPEP) is a 150-170 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. CD13 is a metalloprotease that preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 also has a role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is expressed by granulocytes, monocytes, myeloid leukemia cells and is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas. CD13 functions as an aminopeptidase enzyme and is also alpha receptor for coronavirus an important cause of upper respiratory tract infections.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ANPEP using anti-ANPEP antibody (A02591-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANPEP antigen affinity purified polyclonal antibody (Catalog # A02591-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANPEP at approximately 150KD. The expected band size for ANPEP is at 150KD.
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