Anti-CD134 [OX86]

Absolute Antibody

Anti-CD134 [OX86]

7

This antibody recognises murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes. It is a member of TNFR-superfamily of receptors and acts as a costimulatory molecule involved in long-term T-cell immunity.

OX86 antibody, in its original rat IgG1 format, acts as an OX40 agonist in an Fc-dependent manner and has been widely used in studies investigating the role of OX40 in tumor inhibition (Hirschhorn-Cymerman et al., 2009). Studies comparing two recombinant murine Fc versions (IgG1 and IgG2a) elucidated the importance of the FcR engagement in the antitumor activity of this antibody; the study demonstrated that a stronger murine Fc (in an IgG2a version) was more effective in inducing a CT26 syngeneic tumor regression as well as it led to a stronger Treg depletion in the tumor in comparison with a murine IgG1 version (Metzger et al., 2016). In another study two versions of the OX86 antibody (with a fully functional Fc fragment and with an impaired Fc) and showed that Fc-dependent activity was necessary in inducing systemic antitumor activity (Sagiv-Barfi et al., 2018). FC: This antibody was also used in flow cytometry to stain mouse CD4 and CD8 single-positive cells (al-Shamkhani et al., 1996). FC: Blanquiceth et al. (2016) used this antibody in flow cytometry as well to analyse cells coming from mediastinal lymph nodes and lung tissue. IHC: OX86 antibody was utilised in immunohistochemistry staining of gut tissue (Peyers patches and lamina propria samples) in order to identify OX40-positive cells in colitic mice (Higgins et al., 1999).

Flow Cytometry, ImmunoFluorescence, ELISA, Other Application

FC; agonist; IF; ELISA

Research Use Only

Monoclonal

OX86

Tnfrsf4

tumor necrosis factor receptor superfamily, member 4

ACT3; ACT35; CD134; Ly-70; Ox4; Ox40; OX40 antigen; OX40L receptor; tax-transcriptionally activated glycoprotein 1; tumor necrosis factor receptor superfamily member 4; Txgp; Txgp1; TXGP1L

Mouse

IgG2a

Tumor necrosis factor receptor superfamily member 4

This is a chimeric antibody created to reduce immunogenicity during in vivo applications.

Mouse

Mouse

This antibody was raised against the recombinant mouse OX40 protein.

-20°C,2°C to 8°C

12352203