Anti-CD134 [OX86]

Absolute Antibody

Anti-CD134 [OX86]

7

This antibody recognises murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes. It is a member of TNFR-superfamily of receptors and acts as a costimulatory molecule involved in long-term T-cell immunity.

OX86 antibody, in its original rat IgG1 format, acts as an OX40 agonist in an Fc-dependent manner and has been widely used in studies investigating the role of OX40 in tumor inhibition (Hirschhorn-Cymerman et al., 2009). Studies comparing two recombinant murine Fc versions (IgG1 and IgG2a) elucidated the importance of the FcR engagement in the antitumor activity of this antibody; the study demonstrated that a stronger murine Fc (in an IgG2a version) was more effective in inducing a CT26 syngeneic tumor regression as well as it led to a stronger Treg depletion in the tumor in comparison with a murine IgG1 version (Metzger et al., 2016). In another study two versions of the OX86 antibody (with a fully functional Fc fragment and with an impaired Fc) and showed that Fc-dependent activity was necessary in inducing systemic antitumor activity (Sagiv-Barfi et al., 2018). FC: This antibody was also used in flow cytometry to stain mouse CD4 and CD8 single-positive cells (al-Shamkhani et al., 1996). FC: Blanquiceth et al. (2016) used this antibody in flow cytometry as well to analyse cells coming from mediastinal lymph nodes and lung tissue. IHC: OX86 antibody was utilised in immunohistochemistry staining of gut tissue (Peyers patches and lamina propria samples) in order to identify OX40-positive cells in colitic mice (Higgins et al., 1999).

Flow Cytometry, ImmunoFluorescence, ELISA, Other Application

FC; agonist; IF; ELISA

Research Use Only

Monoclonal

OX86

Tnfrsf4

tumor necrosis factor receptor superfamily, member 4

ACT3; ACT35; CD134; Ly-70; Ox4; Ox40; OX40 antigen; OX40L receptor; tax-transcriptionally activated glycoprotein 1; tumor necrosis factor receptor superfamily member 4; Txgp; Txgp1; TXGP1L

Rabbit

IgG

Tumor necrosis factor receptor superfamily member 4

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

This antibody was raised against the recombinant mouse OX40 protein.

-20°C,2°C to 8°C

12352203