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Anti-CD138 [OC-46F2]

Research Use Only
Ab02724-10.0
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking
Product group Antibodies
ReactivityHuman
TargetSDC1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD138 [OC-46F2]
  • Delivery Days Customer
    7
  • Antibody Specificity
    This antibody is specific for the extracellular domain of human syndecan-1. Syndecan-1 is a cell membrane protein that, after its shedding by heparanase enzymes, is accumulated in the extracellular matrix of some tumours, e.g. myeloma and lung carcinoma,
  • Application Supplier Note
    The original antibody inhibited vascular maturation and tumour growth in an experimental human melanoma model. The therapeutic efficacy of this antibody was also demonstrated in an experimental ovarian carcinoma model. OC-46F2 scFv version was used as a capture antibody in a solid phase sandwich ELISA (PMID: 26460958). The scFv version of this antibody was also used in the immunofluorescent and immunohistochemical analysis of melanoma tissues, human ovarian carcinomas, human normal ovary cells and SKMEL28 cells (PMID: 26460958, 26460958). Syndecan-1 expression on a panel of cell lines was analysed by flow cytometry using recombinant OC-46F2. The scFv antibody can also recognize purified human recombinant extracellular portion of syndecan-1 in a western blot assay. Mice treated with OC-46F2 scFv showed no co-distribution of syndecan-1 with vascular endothelial growth factor receptor 2 (VEGFR2), which is generally seen in intratumour melanoma microenvironment. (PMID: 23352437). Melanoma cells lose their ability to form tubule-like structures in vitro after blocking syndecan-1 using OC-46F2. In a human melanoma xenograft model the combined therapy using OC-46F2 and L19-IL2, an immunocytokine specific for the tumor angiogenic-associated B-fibronectin isoform(B-FN), led to a complete inhibition of tumor growth until day 90 from tumor implantation in 71% of treated mice (PMID: 26460958). The small immuno protein version of this antibody 46F2SIP was used in combination with L19-IL2 for combination therapy in a human ovarian carcinoma model (PMID: 31443604).
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking
  • Applications Supplier
    ELISA; IF; IHC; FC; WB; block
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OC-46F2
  • Gene ID6382
  • Target name
    SDC1
  • Target description
    syndecan 1
  • Target synonyms
    CD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; SDC; SYND1; syndecan; syndecan proteoglycan 1; syndecan-1
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP18827
  • Protein Name
    Syndecan-1
  • Scientific Description
    This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by selecting a human antibody phage display library from human melanoma cells and later on its by its expression in mammalian cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203