Figure 1. Western blot analysis of CD16/Fcgr3 using anti-CD16/Fcgr3 antibody (A01408-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1:mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD16/Fcgr3 antigen affinity purified polyclonal antibody (Catalog # A01408-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD16/Fcgr3 at approximately 70KD. The expected band size for CD16/Fcgr3 is at 70KD.
Figure 1. Western blot analysis of CD16/Fcgr3 using anti-CD16/Fcgr3 antibody (A01408-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1:mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD16/Fcgr3 antigen affinity purified polyclonal antibody (Catalog # A01408-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD16/Fcgr3 at approximately 70KD. The expected band size for CD16/Fcgr3 is at 70KD.
Anti-CD16/Fcgr3 Antibody Picoband(r)
A01408-2-CARRIER-FREE
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetFcgr3
Overview
- SupplierBoster Bio
- Product NameAnti-CD16/Fcgr3 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID14131
- Target nameFcgr3
- Target descriptionFc receptor, IgG, low affinity III
- Target synonymsCD16, low affinity immunoglobulin gamma Fc region receptor III, Fc gamma receptor III, Fcg receptor III, FcgammaRIII, fc-gamma RIII, fcRIII, igG Fc receptor III
- HostRabbit
- IsotypeIgG
- Protein IDP08508
- Protein NameLow affinity immunoglobulin gamma Fc region receptor III
- Scientific DescriptionBoster Bio Anti-CD16/Fcgr3 Antibody Picoband® catalog # A01408-2. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203