Anti-CD19 Antibody-Biotin labled

Biorbyt

Anti-CD19 Antibody-Biotin labled

10

1. Take 100 microl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 microl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Flow Cytometry, ImmunoFluorescence

10 μl / assay FC, IF

Research Use Only

Monoclonal

CB19

Biotin

CD19

CD19 molecule

B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, T-cell surface antigen Leu-12, differentiation antigen CD19

Mouse

B-lymphocyte antigen CD19

Mouse monoclonal antibody against CD19 conjugated to Biotin

Human

2°C to 8°C

12352203