Anti-CD19 [B4]

Absolute Antibody

Anti-CD19 [B4]

7

This antibody reacts with the B4 antigen present on the peripheral blood cells and lymphoid organs, on cell lines derived from normal and malignant B cells, and on tumor cells isolated from patients with B cell-derived neoplasms

This antibody is recommended to be used for the cell surface expression of the B4 antigen. It is reported that in mitogen stimulated B cells the expression of the B4 antigen increases initially with the B cell activation and then is lost with the B cell differentiation (PMID: 6408173). Conjugate of DC1 with murine and humanizsed versions of anti-B4 antibody was found to be potent and effective in selective killing of target cells in culture. It is also reported that the anti-B4-DC1 conjugate showed antitumor efficacy in an aggressive metastatic human B-cell lymphoma survival model in SCID mice and completely cured animals hearing large tumors (PMID: 7545085). Anti-B4 when conjugated with blocked ricin increased the antibody induced cell calcium mobilization and CD19 internalization. Modulation of CD19 induced by anti-B4 or anti-B4-bR was evaluated by indirect immunofluoresecence and flow cytometry (PMID: 9310487). Anti-B4-blocked ricin (anti-B4-bR) recognizes CD19-positive cells, which includes most B-cell non-Hodgkins lymphomas (NHLs). Phase II clinical studies of anti-B4-bR suggested that it does not actively penetrate in certain sites of the disease and is not an active agent in relapsed NHLs (PMID: 9829722)

Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry

IF; FC; IHC

Research Use Only

Monoclonal

B4

CD19

CD19 molecule

B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CVID3; differentiation antigen CD19; T-cell surface antigen Leu-12

Human

IgG1

B-lymphocyte antigen CD19

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was generated against CD19 antigen present on human B cells.

-20°C,2°C to 8°C

12352203