Anti-CD19 [FMC63]

Absolute Antibody

Anti-CD19 [FMC63]

7

This antibody is specific for human CD19, a 95 kDa protein expressed in the B-lymphocyte lineage.

FMC63 was used for four years as a CD19 antibody in various labs prior to its original publication. There, it was reported that the antibody percipitates CD19 and inhibits the binding of other CD19 antibodies to it (Zola et al., 1991; PMID: 1725979). FMC63 was used for IF and FACS of peripheral blood mononuclear cells (PBMC) from patients with multiple myeloma (MM) (Szczepek et al., 1997; PMID: 9057669) and receptor for hyaluronan (HA)-mediated motility (RHAMM) expressing malignant B-lineage cells in myeloma (Crainie et al., 1999; PMID: 10029598). FMC63 was used for IHC and IIP of B cells in lymphocyte-filled villi and IIP of jejunal isolated lymphoid follicle, respectively, in small bowel specimens from human patients (Moghaddami et al., 1998; PMID: 9834269). FNC63 was used to induce B cell depletion in vivo (DiLillo et al., 2011; PMID: 21248259). FMC63 has been incorporated into a CD19 scFv- 41BB- CD3aeta fusion protein-encoding lentiviral vector, which was used in combination with a UCART19 cell therapy to cure high-risk CD19+ infant acute lymphoblastic leukemia (Qasim et al., 2017). This clone did not compete with clone HD37 (available as Ab00214) (Sakemura et al., 2023; PMID: 37879074).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry, Other Application

IF; FC

Research Use Only

Monoclonal

FMC63

CD19

CD19 molecule

B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CVID3; differentiation antigen CD19; T-cell surface antigen Leu-12

Human

IgG1

B-lymphocyte antigen CD19

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

Human prolymphocytic leukaemia cell line JVM3.

-20°C,2°C to 8°C

12352203