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Anti-CD19 [MB19-1]

Ab02364-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
Product group Antibodies
TargetCd19
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD19 [MB19-1]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This clone can be used to detect and analyze the presence of the CD19 transmembrane protein. The specificity towards the protein has been confirmed using ELISA and immunofluorescence (Sato et al., 1996; PMID: 8906812). Additionally, the MB19-1 antibody has been used in several flow cytometry assays. One group used this antibody to determine the differences in CD19 distribution between cells of young and old mice (Yamamoto et al., 2018; PMID: 29625072). Another group used it to differentiate eosinophils from other leukocytes (Vanella et al., 2017; PMID: 27043413). A final group used flow cytometry to determine the fraction of B cells cells in a sample with this antibody (Bedaret et al., 2009; PMID: 19907079).
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA
  • Applications Supplier
    FC; IF; ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MB19-1
  • Gene ID12478
  • Target name
    Cd19
  • Target description
    CD19 antigen
  • Target synonyms
    AW495831; B-lymphocyte antigen CD19; differentiation antigen CD19
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP25918
  • Protein Name
    B-lymphocyte antigen CD19
  • Scientific Description
    This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgA format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Mouse, Dog
  • Reactivity Supplier Note
    This antibody was raised by immunising CD19-deficient mice with a cell line of mCD19+ mouse pre-B cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates, Lane 5: human K562 whole cell lysates, Lane 6: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
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