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ProductsBack/Anti-CD20 [10F381 (rituximab)]

Anti-CD20 [10F381 (rituximab)]

AB00126-16.0-BT
Absolute Antibody
ApplicationsFlow Cytometry
Product group Antibodies
TargetMS4A1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD20 [10F381 (rituximab)]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10F381 (rituximab)
  • Gene ID931
  • Target name
    MS4A1
  • Target description
    membrane spanning 4-domains A1
  • Target synonyms
    B1, Bp35, CD20, CVID5, FMC7, LEU-16, S7, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, CD20 antigen, CD20 receptor, leukocyte surface antigen Leu-16, membrane-spanning 4-domains, subfamily A, member 1
  • Host
    Human
  • Isotype
    IgA1
  • Protein IDP11836
  • Protein Name
    B-lymphocyte antigen CD20
  • Scientific Description
    This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD20/MS4A1 using anti-CD20/MS4A1 antibody (A03780-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD20/MS4A1 antigen affinity purified polyclonal antibody (Catalog # A03780-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD20/MS4A1 at approximately 33KD. The expected band size for CD20/MS4A1 is at 33KD.
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