Anti-CD20 [NCD1.2 (scFv-3)]

Absolute Antibody

Anti-CD20 [NCD1.2 (scFv-3)]

7

The original IgG antibody binds biotinylated canine CD20 peptide with an relative affinity of kd= 340 pM. This antibody was able to recognize endogenous canine CD20 protein in canine 3132 lymphoma cells and also cross reacted with transfected human GFP-CD20 expression construct in H1299 cells in an western blot assay. This antibody can detect canine CD20 expressed on normal peripheral blood, medium-sized B-cell lymphoma and diffuse large B-cell lymphoma in a flow cytometric assay. It could not detect CD20 -negative peripheral T-cell lymphoma cells in flow cytometry. This antibody was also used in the immunohistochemical staining of formalin fixed samples of diffuse large B-cell lymphoma and lymphoma histotypes of B-cell origin, including marginal zone lymphoma, follicular lymphoma, and small lymphocytic lymphoma. (PMID: 26894679)

Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry

WB; IF; FC; IHC

Research Use Only

Monoclonal

NCD1.2 (scFv-3)

MS4A1

membrane spanning 4-domains A1

B1, Bp35, CD20, CVID5, FMC7, LEU-16, S7, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, CD20 antigen, CD20 receptor, leukocyte surface antigen Leu-16, membrane-spanning 4-domains, subfamily A, member 1

Mouse

IgG1

B-lymphocyte antigen CD20

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

41116161