Anti-CD20 [NCD1.2 (scFv-3)]

Absolute Antibody

Anti-CD20 [NCD1.2 (scFv-3)]

7

The original IgG antibody binds biotinylated canine CD20 peptide with an relative affinity of kd= 340 pM. This antibody was able to recognize endogenous canine CD20 protein in canine 3132 lymphoma cells and also cross reacted with transfected human GFP-CD20 expression construct in H1299 cells in an western blot assay. This antibody can detect canine CD20 expressed on normal peripheral blood, medium-sized B-cell lymphoma and diffuse large B-cell lymphoma in a flow cytometric assay. It could not detect CD20 -negative peripheral T-cell lymphoma cells in flow cytometry. This antibody was also used in the immunohistochemical staining of formalin fixed samples of diffuse large B-cell lymphoma and lymphoma histotypes of B-cell origin, including marginal zone lymphoma, follicular lymphoma, and small lymphocytic lymphoma. (PMID: 26894679)

Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry

WB; IF; FC; IHC

Research Use Only

B-lymphocyte antigen CD20

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203