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ProductsBack/Anti-CD204 [2F8 (recombinant version)]

Anti-CD204 [2F8 (recombinant version)]

AB00163-23.0-BT
Absolute Antibody
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetMsr1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD204 [2F8 (recombinant version)]
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2F8 (recombinant version)
  • Gene ID20288
  • Target name
    Msr1
  • Target description
    macrophage scavenger receptor 1
  • Target synonyms
    MRS-A, MSR, MSR-A, SR-A, SR-AI, SR-AII, Scara1, Scvr, macrophage scavenger receptor types I and II, macrophage acetylated LDL receptor I and II, scavenger receptor type A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30204
  • Protein Name
    Macrophage scavenger receptor types I and II
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD204/Msr1 using anti-CD204/Msr1 antibody (A02349-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD204/Msr1 antigen affinity purified polyclonal antibody (Catalog # A02349-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD204/Msr1 at approximately 75-90 kDa. The expected band size for CD204/Msr1 is at 71 kDa.
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