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ProductsBack/Anti-CD226 [FMU-mCD226.1]

Anti-CD226 [FMU-mCD226.1]

Ab03377-8.1
Absolute Antibody
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetCd226
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD226 [FMU-mCD226.1]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The binding of this antibody to recombinant mouse CD226-Fc fusion protein was determined using indirect ELISA. This antibody is capable of binding mouse CD226 expressed on wild-type mouse platelets in a flow cytometric assay. This antibody was also reported to promote platelet aggregation. This antibody can be used to identify CD226 expressed in platelet lysates using western blot (CN111363042).
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    ELISA; FC; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    FMU-mCD226.1
  • Gene ID225825
  • Target name
    Cd226
  • Target description
    CD226 antigen
  • Target synonyms
    DNAM-1, DNAM1, Pta1, TLiSA1, CD226 antigen, platelet and T cell activation antigen 1
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDQ8K4F0
  • Protein Name
    CD226 antigen
  • Scientific Description
    This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    The original antibody was generated by immunizing CD226KO (CD226 knockout) mice with purified CD226-Fc recombinant fusion protein.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD226 using anti-CD226 antibody (A01094-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD226 antigen affinity purified polyclonal antibody (Catalog # A01094-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD226 at approximately 70KD. The expected band size for CD226 is at 70KD.
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ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetCd226
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Absolute Antibody
Anti-CD226 [10E5]AB01366-2.3-VXS
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ReactivityMouse
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