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Immunofluorescent staining of human cell line HEL shows localization to midbody.
Immunofluorescent staining of human cell line HEL shows localization to midbody.
Immunofluorescent staining of human cell line HEL shows localization to midbody.

Anti-CD226 Antibody

HPA050348
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCD226
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CD226
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10666
  • Target name
    CD226
  • Target description
    CD226 molecule
  • Target synonyms
    adhesion glycoprotein; CD226 antigen; DNAM1; DNAM-1; DNAX accessory molecule-1; platelet and T cell activation antigen 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15762
  • Protein Name
    CD226 antigen
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD226 using anti-CD226 antibody (A01094-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD226 antigen affinity purified polyclonal antibody (Catalog # A01094-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD226 at approximately 70 kDa. The expected band size for CD226 is at 70 kDa.
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