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Anti-CD244 [OX-122]

AB00561-7.4-BT
Absolute Antibody
ApplicationsFlow Cytometry, Western Blot, Neutralisation/Blocking
Product group Antibodies
ReactivityMouse
TargetCd244a
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD244 [OX-122]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX-122
  • Gene ID18106
  • Target name
    Cd244a
  • Target description
    CD244 molecule A
  • Target synonyms
    2B4, C9.1, Cd244, F730046O15Rik, Ly90, NAIL, NKR2B4, Nmrk, SLAMF4, natural killer cell receptor 2B4, CD244 natural killer cell receptor 2B4, NK cell receptor 2B4, NK cell type I receptor protein 2B4, SLAM family member 4, non-MHC restricted killing associated, signaling lymphocytic activation molecule 4
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDQ07763
  • Protein Name
    Natural killer cell receptor 2B4
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cd244 using anti-Cd244 antibody (A02527-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse RAW246.7 whole cell lysates, Lane 3: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd244 antigen affinity purified polyclonal antibody (Catalog # A02527-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd244 at approximately 50KD. The expected band size for Cd244 is at 42KD.
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