
Figure 1. Western blot analysis of CD252 using anti-CD252 antibody (A02554-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse CD252 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD252 antigen affinity purified polyclonal antibody (Catalog # A02554-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD252 at approximately 21-25KD. The expected band size for CD252 is at 18KD.
Anti-CD252/Tnfsf4 Antibody Picoband(r)
A02554-1-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetTnfsf4
Overview
- SupplierBoster Bio
- Product NameAnti-CD252/Tnfsf4 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID22164
- Target nameTnfsf4
- Target descriptiontumor necrosis factor (ligand) superfamily, member 4
- Target synonymsAth; Ath-; Ath1; Ath-1; atherosclerosis 1; CD134; CD134L; gp3; gp34; OX4; OX40 ligand; Ox40l; OX-40L; tax-transcriptionally activated glycoprotein 1 ligand; Tnlg2b; tumor necrosis factor ligand 2b; tumor necrosis factor ligand superfamily member 4; Txgp; TXGP1; Txgp1l
- HostRabbit
- IsotypeIgG
- Protein IDP43488
- Protein NameTumor necrosis factor ligand superfamily member 4
- Scientific DescriptionBoster Bio Anti-CD252/Tnfsf4 Antibody Picoband® catalog # A02554-1. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203