Anti-CD257 (BAFF) [Tabalumab]

Absolute Antibody

Anti-CD257 (BAFF) [Tabalumab]

7

Tabalumab neutralises soluble and membrane-bound BAFF, inhibiting BAFF binding to BR3, TACI and BCMA.

Tabalumab has been tested in patients with systemic lupus erythematosus (Merrill 2015) and in patients with active rheumatoid arthritis with an inadequate response to TNF inhibitors (Genovese 2013).

Functional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking

WB; FC; IP; ELISA; Neutralisation; IF; Functional Studies

Research Use Only

Monoclonal

Tabalumab

TNFSF13B

TNF superfamily member 13b

ApoL related ligand TALL-1; BAFF; B-cell-activating factor; B-lymphocyte stimulator; BLYS; CD257; delta BAFF; Delta4 BAFF; dendritic cell-derived TNF-like molecule; DTL; epididymis secretory sperm binding protein; TALL1; TALL-1; THANK; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF20; TNLG7A; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4

Human

IgG1

Tumor necrosis factor ligand superfamily member 13B

This reformatted human antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.

Human, Monkey, Rabbit

Human, Cynomolgus Monkey, Rabbit

Monoclonal antibodies were generated by immunizing human immunoglobulin transgenic mice with soluble human BAFF (amino acids 133–285). Eight mice producing serum antibody titers to human BAFF were injected intravenously with 10 ug human BAFF in phosphate buffered saline. The spleen was harvested 3 days later from each mouse and fused with myeloma cells. 20 Hybridomas were tested for specific binding to human BAFF and to make sure they were expressing human immunoglobulin heavy and light chains by enzyme-linked immunosorbent assay (ELISA). The hybridoma producing the antibody that eventually became tabalumab was a human IgG1 isotype. The antibody was then engineered to a human IgG4 isotype for complete neutralization of BAFF without depletion of BAFF-expressing cells.

-20°C,2°C to 8°C

12352203