Anti-CD257 (BAFF) [Tabalumab]

Absolute Antibody

Anti-CD257 (BAFF) [Tabalumab]

7

ELISA

WB; FC; IP; ELISA; Neutralisation; IF; Functional Studies

Research Use Only

Tabalumab

Human

IgG1

Human, Monkey, Rabbit

Human, Cynomolgus Monkey, Rabbit

Monoclonal antibodies were generated by immunizing human immunoglobulin transgenic mice with soluble human BAFF (amino acids 133–285). Eight mice producing serum antibody titers to human BAFF were injected intravenously with 10 ug human BAFF in phosphate buffered saline. The spleen was harvested 3 days later from each mouse and fused with myeloma cells. 20 Hybridomas were tested for specific binding to human BAFF and to make sure they were expressing human immunoglobulin heavy and light chains by enzyme-linked immunosorbent assay (ELISA). The hybridoma producing the antibody that eventually became tabalumab was a human IgG1 isotype. The antibody was then engineered to a human IgG4 isotype for complete neutralization of BAFF without depletion of BAFF-expressing cells.

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

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